How to conduct in vitro expansion of mouse splenocytes? There are various methods and the one you choose will depend on your resources, your budget and the exact results you need. For this article I am concentrating on how to expand them in the lab to get better quality cells for ongoing research. The procedure is relatively painless but can be time consuming with repeated visits for autologous injections.
Mice are used in many cell culture and research projects. Mice are also excellent for studying many diseases and disorders because of their speed and efficiency in growth assays. They can also be used to study cancer treatments and the response of tissues to specific therapies. These traits make them an ideal and cost-effective model for researchers and doctors alike.
Mice can be easily infected by viruses like MLTV and Q-ml and they succumb to infections very quickly.
Therefore it is necessary to control any infections prior to and during culture. This can be achieved using Erythromycin and can be done monthly or every three weeks. Mice are also susceptible to growth arrest due to reduced splenic capacity. Therefore, additional doses are needed to bring up cell counts.
The method for how to conduct in vitro expansion of mouse splenocytes? One method is through transvaginal laser ablation, where the laser energy is used to vaporize abnormal cells. The normal cells are then purified through electrodessication, washing and infection. The procedure is usually successful in improving the quality and number of normal cells but sometimes produces mixed results. Some of the mixed results may include increased sensitivity to insulin and impaired glucose tolerance.
One of the best ways is to use the growth arrest assay.
It is a highly sensitive test for detecting insulin resistant tumor cells. This works by injecting glucose in a petri dish and then looking for evidence of growth arrest. If abnormal cells are found, they are then tested for insulin secretion.
Another procedure is known as v-erboyalty transfer. This involves splenocyte proliferation in a non cancerous organ such as the kidney. Mice are genetically programmed to prefer a red blood cell and so the kidney is an ideal organ to test this procedure. When a mouse is given a preference for one type of cell over another, the scientists can then inject a particular protein into the mice. When this occurs, the mice will start to grow and develop normally.